Molecular characterization of Cry1D-133 toxin from Bacillus thuringiensis strain HD133 and its toxicity against Spodoptera littoralis.
Identifieur interne : 000433 ( Main/Exploration ); précédent : 000432; suivant : 000434Molecular characterization of Cry1D-133 toxin from Bacillus thuringiensis strain HD133 and its toxicity against Spodoptera littoralis.
Auteurs : Dalel Benfarhat-Touzri [Tunisie] ; Fatma Driss [Tunisie] ; Sonia Jemli [Tunisie] ; Slim Tounsi [Tunisie]Source :
- International journal of biological macromolecules [ 1879-0003 ] ; 2018.
Descripteurs français
- KwdFr :
- Animaux (MeSH), Bacillus thuringiensis (génétique), Bacillus thuringiensis (métabolisme), Endotoxines (génétique), Endotoxines (métabolisme), Endotoxines (pharmacologie), Hémolysines (génétique), Hémolysines (métabolisme), Hémolysines (pharmacologie), Larve (génétique), Larve (microbiologie), Lutte biologique contre les nuisibles (MeSH), Protéines bactériennes (génétique), Protéines bactériennes (métabolisme), Protéines bactériennes (pharmacologie), Spodoptera (effets des médicaments et des substances chimiques), Spodoptera (microbiologie), Spodoptera (pathogénicité), Toxines bactériennes (pharmacologie).
- MESH :
- effets des médicaments et des substances chimiques : Spodoptera.
- génétique : Bacillus thuringiensis, Endotoxines, Hémolysines, Larve, Protéines bactériennes.
- microbiologie : Larve, Spodoptera.
- métabolisme : Bacillus thuringiensis, Endotoxines, Hémolysines, Protéines bactériennes.
- pathogénicité : Spodoptera.
- pharmacologie : Endotoxines, Hémolysines, Protéines bactériennes, Toxines bactériennes.
- Animaux, Lutte biologique contre les nuisibles.
English descriptors
- KwdEn :
- Animals (MeSH), Bacillus thuringiensis (genetics), Bacillus thuringiensis (metabolism), Bacillus thuringiensis Toxins (MeSH), Bacterial Proteins (genetics), Bacterial Proteins (metabolism), Bacterial Proteins (pharmacology), Bacterial Toxins (pharmacology), Endotoxins (genetics), Endotoxins (metabolism), Endotoxins (pharmacology), Hemolysin Proteins (genetics), Hemolysin Proteins (metabolism), Hemolysin Proteins (pharmacology), Larva (genetics), Larva (microbiology), Pest Control, Biological (MeSH), Spodoptera (drug effects), Spodoptera (microbiology), Spodoptera (pathogenicity).
- MESH :
- chemical , genetics : Bacterial Proteins, Endotoxins, Hemolysin Proteins.
- chemical , metabolism : Bacterial Proteins, Endotoxins, Hemolysin Proteins.
- chemical , pharmacology : Bacterial Proteins, Bacterial Toxins, Endotoxins, Hemolysin Proteins.
- chemical : Bacillus thuringiensis Toxins.
- drug effects : Spodoptera.
- genetics : Bacillus thuringiensis, Larva.
- metabolism : Bacillus thuringiensis.
- microbiology : Larva, Spodoptera.
- pathogenicity : Spodoptera.
- Animals, Pest Control, Biological.
Abstract
Bacillus thuringiensis subsp. aizawai strain HD133, known by its effectiveness against Spodoptera species, produces bipyramidal crystals encompassing the insecticidal proteins Cry1Ab, Cry1Ca and Cry1D-133 in the proportions 60:37:3, respectively. In this study, we dealt with the relevance of the low rate of Cry1D-133. The cry1D-133 gene from HD133 was cloned and sequenced. Both nucleotide and amino acid sequence similarity analyses with the cry1D genes available in the GenBank database revealed that cry1D-133 is a new variant of cry1Da-type genes with 99% identity with cry1Da1. Molecular modeling of the Cry1D-133 toxin showed that its higher toxicity is correlated to a higher number of toxin-receptor interactions. Optimal culture conditions of 4 h post-induction time, 160 rpm agitation and 37 °C post-induction temperature were determined and adopted to overproduce Cry1D-133 toxin at adequate amounts to carryout bioassays. A gradual increase of the proportion of Cry1D-133 to the HD133 insecticidal proteins forming the crystal (Cry1D-133, Cry1Ca and Cry1Ab) showed an improvement of the toxicity against Spodoptera littoralis larvae. Therefore, the potential of Cry1D-133 to enhance HD133 toxicity could promote its combination with other B. thuringiensis insecticidal proteins toxins in order to increase target range or to delay the emergence of resistance.
DOI: 10.1016/j.ijbiomac.2018.01.081
PubMed: 29366893
Affiliations:
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Box, "1177", 3018, Sfax, Tunisia.</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>Laboratory of Biopesticides, Centre of Biotechnology of Sfax, University of Sfax, P.O. Box, "1177", 3018, Sfax</wicri:regionArea><wicri:noRegion>Sfax</wicri:noRegion></affiliation></author><author><name sortKey="Driss, Fatma" sort="Driss, Fatma" uniqKey="Driss F" first="Fatma" last="Driss">Fatma Driss</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Biopesticides, Centre of Biotechnology of Sfax, University of Sfax, P.O. Box, "1177", 3018, Sfax, Tunisia. Electronic address: driss.fatma@cbs.rnrt.tn.</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>Laboratory of Biopesticides, Centre of Biotechnology of Sfax, University of Sfax, P.O. Box, "1177", 3018, Sfax</wicri:regionArea><wicri:noRegion>Sfax</wicri:noRegion></affiliation></author><author><name sortKey="Jemli, Sonia" sort="Jemli, Sonia" uniqKey="Jemli S" first="Sonia" last="Jemli">Sonia Jemli</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Microbial Biotechnology and Enzyme Engineering, Centre of Biotechnology of Sfax, University of Sfax, P.O. Box, "1177", 3018, Sfax, Tunisia.</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>Laboratory of Microbial Biotechnology and Enzyme Engineering, Centre of Biotechnology of Sfax, University of Sfax, P.O. Box, "1177", 3018, Sfax</wicri:regionArea><wicri:noRegion>Sfax</wicri:noRegion></affiliation></author><author><name sortKey="Tounsi, Slim" sort="Tounsi, Slim" uniqKey="Tounsi S" first="Slim" last="Tounsi">Slim Tounsi</name><affiliation wicri:level="1"><nlm:affiliation>Laboratory of Biopesticides, Centre of Biotechnology of Sfax, University of Sfax, P.O. Box, "1177", 3018, Sfax, Tunisia.</nlm:affiliation><country xml:lang="fr">Tunisie</country><wicri:regionArea>Laboratory of Biopesticides, Centre of Biotechnology of Sfax, University of Sfax, P.O. Box, "1177", 3018, Sfax</wicri:regionArea><wicri:noRegion>Sfax</wicri:noRegion></affiliation></author></analytic><series><title level="j">International journal of biological macromolecules</title><idno type="eISSN">1879-0003</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term><term>Bacillus thuringiensis (genetics)</term><term>Bacillus thuringiensis (metabolism)</term><term>Bacillus thuringiensis Toxins (MeSH)</term><term>Bacterial Proteins (genetics)</term><term>Bacterial Proteins (metabolism)</term><term>Bacterial Proteins (pharmacology)</term><term>Bacterial Toxins (pharmacology)</term><term>Endotoxins (genetics)</term><term>Endotoxins (metabolism)</term><term>Endotoxins (pharmacology)</term><term>Hemolysin Proteins (genetics)</term><term>Hemolysin Proteins (metabolism)</term><term>Hemolysin Proteins (pharmacology)</term><term>Larva (genetics)</term><term>Larva (microbiology)</term><term>Pest Control, Biological (MeSH)</term><term>Spodoptera (drug effects)</term><term>Spodoptera (microbiology)</term><term>Spodoptera (pathogenicity)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux (MeSH)</term><term>Bacillus thuringiensis (génétique)</term><term>Bacillus thuringiensis (métabolisme)</term><term>Endotoxines (génétique)</term><term>Endotoxines (métabolisme)</term><term>Endotoxines (pharmacologie)</term><term>Hémolysines (génétique)</term><term>Hémolysines (métabolisme)</term><term>Hémolysines (pharmacologie)</term><term>Larve (génétique)</term><term>Larve (microbiologie)</term><term>Lutte biologique contre les nuisibles (MeSH)</term><term>Protéines bactériennes (génétique)</term><term>Protéines bactériennes (métabolisme)</term><term>Protéines bactériennes (pharmacologie)</term><term>Spodoptera (effets des médicaments et des substances chimiques)</term><term>Spodoptera (microbiologie)</term><term>Spodoptera (pathogénicité)</term><term>Toxines bactériennes (pharmacologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Bacterial Proteins</term><term>Endotoxins</term><term>Hemolysin Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Bacterial Proteins</term><term>Endotoxins</term><term>Hemolysin Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Bacterial Proteins</term><term>Bacterial Toxins</term><term>Endotoxins</term><term>Hemolysin Proteins</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Bacillus thuringiensis Toxins</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Spodoptera</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Spodoptera</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Bacillus thuringiensis</term><term>Larva</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Bacillus thuringiensis</term><term>Endotoxines</term><term>Hémolysines</term><term>Larve</term><term>Protéines bactériennes</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Bacillus thuringiensis</term></keywords><keywords scheme="MESH" qualifier="microbiologie" xml:lang="fr"><term>Larve</term><term>Spodoptera</term></keywords><keywords scheme="MESH" qualifier="microbiology" xml:lang="en"><term>Larva</term><term>Spodoptera</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Bacillus thuringiensis</term><term>Endotoxines</term><term>Hémolysines</term><term>Protéines bactériennes</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Spodoptera</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Spodoptera</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Endotoxines</term><term>Hémolysines</term><term>Protéines bactériennes</term><term>Toxines bactériennes</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Pest Control, Biological</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Lutte biologique contre les nuisibles</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Bacillus thuringiensis subsp. aizawai strain HD133, known by its effectiveness against Spodoptera species, produces bipyramidal crystals encompassing the insecticidal proteins Cry1Ab, Cry1Ca and Cry1D-133 in the proportions 60:37:3, respectively. In this study, we dealt with the relevance of the low rate of Cry1D-133. The cry1D-133 gene from HD133 was cloned and sequenced. Both nucleotide and amino acid sequence similarity analyses with the cry1D genes available in the GenBank database revealed that cry1D-133 is a new variant of cry1Da-type genes with 99% identity with cry1Da1. Molecular modeling of the Cry1D-133 toxin showed that its higher toxicity is correlated to a higher number of toxin-receptor interactions. Optimal culture conditions of 4 h post-induction time, 160 rpm agitation and 37 °C post-induction temperature were determined and adopted to overproduce Cry1D-133 toxin at adequate amounts to carryout bioassays. A gradual increase of the proportion of Cry1D-133 to the HD133 insecticidal proteins forming the crystal (Cry1D-133, Cry1Ca and Cry1Ab) showed an improvement of the toxicity against Spodoptera littoralis larvae. Therefore, the potential of Cry1D-133 to enhance HD133 toxicity could promote its combination with other B. thuringiensis insecticidal proteins toxins in order to increase target range or to delay the emergence of resistance.</div></front></TEI><affiliations><list><country><li>Tunisie</li></country></list><tree><country name="Tunisie"><noRegion><name sortKey="Benfarhat Touzri, Dalel" sort="Benfarhat Touzri, Dalel" uniqKey="Benfarhat Touzri D" first="Dalel" last="Benfarhat-Touzri">Dalel Benfarhat-Touzri</name></noRegion><name sortKey="Driss, Fatma" sort="Driss, Fatma" uniqKey="Driss F" first="Fatma" last="Driss">Fatma Driss</name><name sortKey="Jemli, Sonia" sort="Jemli, Sonia" uniqKey="Jemli S" first="Sonia" last="Jemli">Sonia Jemli</name><name sortKey="Tounsi, Slim" sort="Tounsi, Slim" uniqKey="Tounsi S" first="Slim" last="Tounsi">Slim Tounsi</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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